ESEL Paper Review_20140324
By Hong Guo
Phone: (+82) (0)10 82276568
1, Title and Author
Title: Unstructured Model for L-Lysine Fermentation under Controlled Dissolved
Semsi Ensari*,†, Joon Ha‡ , and Henry C.Lim‡
Biotechnology Development, Schering-Plough Research Institute, 1011 Morris Avenue, U-14-2-20,Union, New Jersey 07083
Department of Chemical Engineering and Materials Science, University of
California-Irvine, Irvine, California 92697
2. Summary of Paper
? In this study, an unstructured model was developed for batch cultivation of Corynebacterium lactofermentum under controlled dissolved oxygen.
? The specific rates developed using batch culture data are adapted for fed-batch cultures and an intuitive optimization technique is used to show through simulation superiority of fed-batch operation
? The overall cell yield drops significantly at high initial glucose concentrations, while the overall product yield appears total level off .
? The productivity of the continuous culture is 60% higher than the batch culture, and both the cell and the product yields are comparable to batch data.
? While low productivity is inherent in comparing batch against continuous cultures, batch culture produced a higher amount of product due to almost complete consumption of residual substrate.
? Fed-Batch Culture.
? Finally, to simulate fed-batch culture, the initial conditions were set to 0.1 g/L cell, optimal substrate concentration, S* ) 17.1 g/L, no product, and a bioreactor volume of 3 L. The feed substrate concentration, SF, final bioreactor volume, Vf, and maximum flow rate, Fmax, were set to 400 g/L, 10 L and 360 mL/h, respectively.
? The simulations were stopped when the substrate concentration was reduced to less than 1 g/L. Total cultivation times for batch and fed-batch cultivations were found to be 284 and 243 h, respectively.
? In comparison to batch culture with a high initial substrate concentration, fed-batch culture with a high initial substrate concentration, fed-batch culture displays a rapid increases in cell and product concentration.
? Compared to batch culture, the overall cell yield decreases and the product yield increases.
This research would be fully studied and applied to the research of reactor modelling, which is related to the our current class- Environmental Systems Engineering.
5. Contact (Mail address): email@example.com.